스트레스가 Streptozotocin유도 당뇨백서의 타액선에 미치는 영향
The Effect of Salivary Gland of Streptozotocin Induced Diabetic Rats by Stress
김흥모, 전양현, 진용옥,
소속 상세정보
김흥모 ( ) - 경희대학교 치과대학 구강내과학
전양현 ( ) - 경희대학교 치과대학 구강내과학
진용옥 ( ) - 경희대학교 치과대학 구강내과학
KMID : 0355219970220010065
Abstract
Cytochrome P450 is an oxidase involved in oxidation of alcohol and is known to be an activator of carcinogen.
The present study was perfomed to analyze the effect of diabetes and cold stress on the expression of Cytochrome P450 IE1(CYPIIEI) in the liver and salivary glands in rats by an imm umoblot analysis.
Sixty three were divided into 4 groups; 1) 20 rats belonging to group I were allowed diabetes(40mg/kg. LV.) 2) 20 rats of group II were bathed in cold water for 30 seconds twice a day 3) 20 rats comprising group III were received diabetes and cold ´stress as described above 4) 3 rats of group N were selected as a control.
The rats were sacrificed at the end of the same day 1, 2, 3, 4weeks experimet. The liver and parotid glands were removed and stored at -70°C until use. The stored organs were homogenized for 10 seconds and the supernatants were obtained by centrifugation. The proteins of the supernatants were separated by - sodium dodecyl sulfate-polyacryiarmde gel elechnphbresis and subjected to Western blotting. The blotted membranes were incubated with polyclonal antibodies to CYPIIEI.
And specimens were observed with light microscope also under the Hematoxillin-Eosin staining.
The obtained results were as follows
1. In diabetes group, acini had changed to degeneration severly lweek after experiment; but repaired gradually in lapse of time.
2 In diabetes group, septa] connective tissues had changed to degeneration little by little from lweek after experiment, and progressed severely in lapse of time.
3. In stress group, acini had not changed remarkably, but slightly separated each other 3weeks after experiment.
4. In diabetes and stress group, histological feature had changed remarkably campare with in the group of diabetes only.
5. In all experimental group, CYPIIE1 had expressed remarkably in the liver tissues, but not in the parotid gland tissues.
6. In diabetes and stress group, CYPIIE1 had expressed remarkably campare with in the group of diabetes only.
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